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Efficient genetic transformation of cotyledonary explants of tomato (Solanum lycopersicum, cv. H-86, Kashivishesh) was obtained. Disarmed Agrobacterium tumifaciens strain GV 3101 was used in conjugation withbinary vector pBinAR containing a construct consisting of the coding sequence of the BcZAT12 gene underthe regulatory control of the stress inducible Bclea1a promoter. ZAT12 encodes a C2H2 zinc finger proteinwhich confers multiple abiotic stress tolerance to plants. Integration of ZAT12 gene into nuclear genome ofindividual kanamycin resistant transformed To tomato lines was confirmed by Southern blot hybridizationwith segregation analysis of T1 plants showing Mendelian inheritance of the transgene. Expressionof ZAT12 in drought-stressed transformed tomato lines was verified in T2 generation plants using RT-PCR.Of the six transformed tomato lines (ZT1–ZT6) the transformants ZT1 and ZT5 showed maximum expressionof BcZAT12 gene transcripts when exposed to 7 days drought stress. Analysis of relative water content(RWC), electrolyte leakage (EL), chlorophyll colour index (CCI), H2O2 level and catalase activity suggestedthat tomato BcZAT12 transformants ZT1 and ZT5 have significantly increased levels of drought tolerance.These results suggest that BcZAT12 transformed tomato cv. H-86 has real potential for molecular breedingprograms aimed at augmenting yield of tomato in regions affected with drought stress. 2012 Elsevier Ltd. All rights
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