Next generation sequencing (NGS) technologies provide a high-throughput means to generate large amount of sequence
data. However, quality control (QC) of sequence data generated from these technologies is extremely important for
meaningful downstream analysis. Further, highly efficient and fast processing tools are required to handle the large volume
of datasets. Here, we have developed an application, NGS QC Toolkit, for quality check and filtering of high-quality data. This
toolkit is a standalone and open source application freely available at http://www.nipgr.res.in/ngsqctoolkit.html. All the
tools in the application have been implemented in Perl programming language. The toolkit is comprised of user-friendly
tools for QC of sequencing data generated using Roche 454 and Illumina platforms, and additional tools to aid QC
(sequence format converter and trimming tools) and analysis (statistics tools). A variety of options have been provided to
facilitate the QC at user-defined parameters. The toolkit is expected to be very useful for the QC of NGS data to facilitate
better downstream analysis.