The DPPH∙ active EAF (17.2 g) was l

The DPPH∙ active EAF (17.2 g) was loaded as a dried slurry of silica gel to column chromatography (45 × 3.5 cm) and eluted with petroleum ether: EtOAc gradient elution (100 : 0—0 : 100), in increasing order of polarity. A total of 92 fractions of 100mL each were collected and analyzed by TLC (Silica gel F254 plates 20 × 20cm, Merck, Germany). TLC analysis was carried out using ethyl acetate: chloroform: formic acid (5:4:1) as the mobile phase and the separated bands were visualized using iodine vapors and vanillin-sulphuric acid reagent. These fractions were pooled to afford seven major fractions (Fr. A: 1–13, Fr. B: 14–35, Fr. C: 36–48, Fr. D: 49–60, Fr. E: 61–70, Fr. F: 71–80, and Fr. G: 81–92) based on TLC analysis. These fractions were tested for bioactivity using DPPH spectrophotometric assay.
For further purification, the highly active Fr. B (2.8 g) was loaded on a silica gel column, eluted with petroleum ether- EtOAc gradients, and the ethyl acetate content of themixture was increased in a series of 5% steps. The inactive and less active proved fractions were discarded. Finally, the active Fr.B2 eluted with petroleum ether-ethyl acetate (85 : 15) yielded 117mg of compound. The purity of isolated compound was establishedbyHPLCandits structurewas confirmed through the interpretation of the spectral data (UV, FT-IR, 1H, 13C NMR, and MS) and further tested for its antioxidant effects.

Results (English) 3:

DPPH∙活性电弧炉（17.2 g）为干浆硅胶柱层析装（45×3.5厘米），用石油醚：乙酸乙酯梯度洗脱（100：0-0：100），在极性增加的顺序。共92分每100毫升的收集和分析，采用薄层色谱法（硅胶F254板20×20cm，默克，德国）。用乙酸乙酯进行薄层色谱分析：氯仿：甲酸（5:4:1）为流动相，分离带用碘蒸气和香草醛-硫酸试剂可视化。这些馏分被汇集起七个主要部分（果：1–13，Fr. B：14–35，Fr. 36–C：48，Fr. D：49–60，Fr. E：61–70，果期：71–80，Fr. G：81–92）基于薄层色谱分析。这些组分进行了测试使用DPPH法测定生物活性。为进一步净化，非常活跃的Fr. B（2.8克）装上硅胶柱，用石油醚- EtOAc梯度，和混合的乙酸乙酯含量的一系列步骤增加5%。不活跃和不太活跃的证明分数被丢弃。最后，积极fr.b2用石油醚、乙酸乙酯（85∶15）取得了117mg复合。分离的化合物的纯度establishedbyhplcandits结构通过对光谱数据的解释证实（UV，FT-IR，1H，13C NMR，和MS）进一步测试其抗氧化作用。