2. Study of bioethanol production performance of screened yeast. . Divided into four ways
2.1 Preliminary test of bioethanol production
First, inoculate from microtube into 3o mL of YM broth and then bring to centrifuge next added with 0.85% w/v NaCl , After that adjust OD to 1.5 approximately
at 600 nm, then mix yeast solution with YM broth (45% w/v glucose) ( 1: 2 of ratio) and fermentation in Eihorn tube at 40 ˚C and the end gas observation every 12 h.