グアニル酸シクロヒドロラーゼ1酵素(GCH1) はテトラヒドロビオプテ

グアニル酸シクロヒドロラーゼ1酵素(GCH1) はテトラヒドロビオプテリンBH4 生合成経路の制限酵素であり、痛みの神経伝導に関与する調節因子の生成を制御する重要な役割を果たしている。近年の研究によりGCH1が疼痛の新しい制御因子であることは明らかとなった。一方GCH１の酵素活性はGFRPという調節因子によって修飾される。今回、免疫組織化学解析、ＰＬＡ及びＢＮ電気泳動を用い、GCH1/ GFRP複合体形成の調節機構を検討した。セロトニン作動性及びドパミン作動性の神経細胞にGCH1/GFRPの共存がみられた。またGCH1/GFRP相互作用は主に感覚神経系に認めた。この結果は新規な鎮痛治療法の開発に寄与するものである

Results (English) 1:

Is the guanylic acid GTP cyclohydrolase 1 enzyme (GCH1) BH4 tetrahydrobiopterin biosynthesis pathway of restriction enzymes, and plays an important role controlling the formation of regulatory factors involved in the pain nerve conduction. GCH1 new control factors of pain by recent studies that concluded. On the other hand GCH1 enzyme activity was qualified by the adjustment factor of GFRP. This time the immune tissue chemical analysis, the PLA and the BN electrophoresis used, considering the regulation of GCH1 / GFRP complex formation. Serotonin, cholinergic and dopaminergic-cholinergic neurons observed to coexist GCH1/GFRP. Also GCH1/GFRP interactions are mainly admitted sensory nervous system. This results in contributing to the development of new pain treatments

Results (English) 2:

Guanylate cyclohydrolase 1 enzyme (GCH1) is a restriction enzyme of tetrahydrobiopterin BH4 biosynthetic pathway, it plays an important role in controlling the generation of regulatory factors involved in the pain of nerve conduction. It became clear GCH1 is by recent studies is a new regulator of pain. On the other hand enzymatic activity of GCH1 it is modified by an adjustment factor of GFRP. This time, immunohistochemical analysis, using PLA and BN electrophoresis to examine the adjustment mechanism GCH1 / GFRP complex formation. Coexistence of GCH1 / GFRP was seen in serotonergic and dopaminergic neurons. Also GCH1 / GFRP interaction we were mainly observed in the sensory nervous system. This result is to contribute to the development of novel analgesic therapies

Results (English) 3:

Guanosine monophosphate cyclohydrolase 1 enzyme GCH 1 and 4 BH restriction of tetrahydrobiopterin synthesis pathway plays an important role to control the generation of regulatory factors involved in pain nerve conduction. According to the recent research GCH 1 is a new factor controlling the pain became clear. On the other hand, the enzyme activity of GCH regulatory factor 1 and GFRP by modification. The immunohistochemical analysis, and PLA BN electrophoresis to study the regulatory mechanism of GCH 1 GFRP complex formation.Serotonergic and there was a GCH GFRP coexistence of dopaminergic neurons. The GCH 1 GFRP interaction mainly in the sensory nervous system was recognized. The results contribute to the development of novel analgesic therapy