Another more recently published article discusses the
impact of spatial sampling density in Raman Imaging
[53]. In this investigation, Raman point mapping was
applied to a fixed (magnified) area of an over-the-counter
drug using spatial steps ranging from 10 to 100 lm. The
coarse grid (with 100 lm steps) Raman image took a few
minutes to acquire, while the fine grid (with 10 lm steps)
took several hours to record. It was demonstrated that
the coarse measurement could be useful for selection of
regions which required further fine measurement. Although
the fine grid method is time consuming, it would allow for
visualisation of the chemical distribution of the sample
with details of crystal morphology.