BLOCKING CELLULAR DIVISION OF ACCES

BLOCKING CELLULAR DIVISION OF ACCESSORY/STIMULATOR CELLS
There are two situations in which inhibition of accessory or stimulator cell division should
be blocked. When purified T cells rather than unfractionated lymphoid populations are
used in Basic Protocol 1, cultures are frequently supplemented with accessory cells
syngeneic to the responder T cells. If accessory cell DNA synthesis is inhibited, one can
then be certain that the resultant proliferative response is comprised entirely of responder
T cells and does not contain a component of recruited B cell proliferation derived from
the accessory cell populations. In the MLR, the stimulator cells are spleen cells from mice
that differ from the responder cells in H-2 and/or Mls gene expression (see APPENDIX 1,
Tables A.1C.1 and A.1F.1) and they can also recognize alloantigens on the responder cells.
This responsiveness of stimulator cells against responder cells in an MLR (so-called
back-stimulation) must be prevented by blocking cellular division. This can be done by

Results (English) 3:

阻止附件/刺激细胞
有两种情况下，副或刺激细胞分裂抑制应该
阻断细胞分裂。当纯化的T细胞而不是普通淋巴人口
用于基本协议1，文化是经常添加辅助细胞
同源的T细胞应答。如果辅助细胞DNA合成受到抑制，可以
就可知道所产生的增殖反应是完全由抢答器
T细胞和不包含组件的B细胞增殖
招募来自附件的细胞群。在国土资源部，刺激细胞从小鼠脾细胞
不同于应答细胞H-2和/或MLS基因表达（见附件1，表1和a.1f
a.1c。。1），他们还可以识别应答细胞同种抗原。
这种反应，刺激细胞与反应细胞在MLR（所谓的
回刺激）必须通过阻断细胞分裂防止。这是可以做到的