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gills 1-2 were small and were combined for assay and homogenized in 0.500 ml HW. gills 3-8 were assay individually and homogennize 0.700 ml HM. protein concentration in the crude homogentes varied between 0.1และ0.5 mg * ml and were measured colorimetrically at 595 nm using the bio-rad protein assay with bovine serum albumin as a standard
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