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Electrospinning techniques have been used to create fibrous scaffolds that mimic the natural ECM for study of in vivo-like phenotypic shape and gene expression (49).Interestingly, the orientation of electrospun fibers is able to provide contact guidance for the attached cells, and has an impact on their morphology and even their differentiation capacity. It has been reported that aligned electrospun fibers promote Schwann cell maturation, neurite outgrowth, vascular endothelial cell growth, and myotube formation (6). The regular alignment of fibers leads to enhanced osteogenic responses in MSCscompared with the fibers having a randomly networked architecture
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