Analytical procedures and statistical analyses
Samples of faeces, chaff, unlabelled-SFM, labelled-SFM and any feed refusals were freeze-dried prior to
chemical analysis (in triplicate). Procedures for the extraction of alkanes from these samples and from
beeswax, and subsequent quantification by gas chromatography, have been described elsewhere (Mayes et al.
1986; Dove and Mayes 1996). Known and estimated total and supplement intakes were compared by linear
regression or by using Students t-test for paired comparisons.