For comparing the human blood serum and drinking water fluoride levels of subjects
with dental fluorosis and bony deformities, this study is carried out with individuals ranging 2-19
age group fluorinated Sham Ki Bhatiyan, Punjab (endemic) and Queens Road, Lahore, Punjab (nonendemic)
areas. Fluoride concentrations were determined using ion selective electrode methodology
and statistically compared. Both the groups showed a significant difference (p < 0.05). Subjects
from fluorotic area showed high concentration of fluoride in water and blood serum samples (mean
value: 135.587±77.435 and 2.765±0.469 μmolL-1 in water and blood serum samples respectively) as
compared to controls (mean value: 19.509±2.432 and 2.364± 0.667 μmolL -1). These findings
indicate that serum and water fluoride concentrations have a significant positive dose response
relationship with the prevalence of dental fluorosis in an area associated with high fluoride level in