Reverse blot hybridisation assayREB

Reverse blot hybridisation assay

REBA Myco-ID was used according to the manufacturer’s instructions to detect M. tuberculosis and NTM in tissue samples from patients with granulomatous ymphadenitis. This assay can differentiate 18 mycobacterial species, including M. tuberculosis, M. avium, M. intracellulare, M. scrofulaceum, M. szulgai, M. terrae, M. chelonae, M. abscessus, M. gordonae, M. kansasii, M. celatum, M. fortuitum, M. ulcerans/marinum,M. genavense/simiae, M. aubagnense and M. mucogenicum,
using specific probes for the rpoB gene region.9,10 Briefl y, the rpoB gene region was amplified with biotin-labelled primers and the PCR products were hybridised to a membrane with 19 immobilised probes. The membrane was incubated with streptoavidin-conjugated alkaline phosphatase and visualised with nitro blue tetrazolium chloride and 5-bromo-4-chloro-3-indolyl phosphate. The data were interpreted using the REBA Myco-ID data sheet provided by the manufacturer.
Mycobacterial drug resistance was determined using a REBA MTB-MDR kit, which detects rifampicin (RMP) and isoniazid (INH) resistance using 13 probes for rpoB, the oxyR-ahpC intergenic region, katG, and the inhA promoter region from M. tuberculosis.

Results (English) 1:

Reverse blot hybridisation assayREBA Myco-ID was used according to the manufacturers instructions to detect M. tuberculosis and NOT TO MUCH in tissue samples from patients with granulomatous ymphadenitis. This assay can differentiate 18 mycobacterial species, including M. tuberculosis, M. avium, M. intracellulare, M. scrofulaceum, M. szulgai, M. terrae, M. chelonae, M. abscessus, M. gordonae, M. kansasii, M. celatum, M. fortuitum, M. ulcerans/marinum, M. genavense/simiae, M. aubagnense and M. mucogenicum,using specific probes for the rpoB gene region. 9, 10 Briefl y, the rpoB gene region was amplified with biotin-labeled primers and the PCR products were hybridised to a membrane with 19 immobilised probes. The membrane was incubated with streptoavidin-conjugated alkaline phosphatase and visualised with nitro blue tetrazolium chloride and 5-bromo-4-chloro-3-indolyl phosphate. The data were interpreted using the REBA Myco-ID data sheet provided by the manufacturer.Mycobacterial drug resistance was determined using a REBA MTB-MDR kit, which detects rifampicin (RMP) and isoniazid (INH) resistance using 13 probes for rpoB, the oxyR-ahpC intergenic region, katG, and the inhA promoter region from M. tuberculosis.

Results (English) 3:

反向斑点杂交法检测该ID

利巴使用根据制造商的指示来检测结核和NTM患者组织标本中的肉芽肿性淋巴结炎。此法可以区分18种分枝杆菌，包括结核分枝杆菌，鸟分枝杆菌，M.杆菌，瘰疬，M.斯塞格，M. terrae，龟龟，M.，M.戈登，M.M.堪萨斯，隐藏，偶发分枝杆菌，溃疡分枝杆菌/海鱼，M.日内瓦/猿，M.和M. aubagnense mucogenicum
，使用特异性探针的rpoB基因区域。9 briefl，rpoB基因区域与生物素标记的引物和PCR扩增产物进行杂交膜19固定探针。膜孵育与链霉亲和素标记的碱性磷酸酶和可视化与氯化硝基四氮唑蓝，发光磷。数据是用利巴该ID数据表由制造商提供的解释。用利巴
mtb-mdr试剂盒检测结核分枝杆菌耐药性，检测利福平（RMP）和异烟肼（INH）使用13探针电阻的rpoB，katG，oxyR ahpC基因间隔区，与inhA基因启动子区结核杆菌。