The device performs sequencing
by DNA synthesis on clusters of identical DNA molecules
simultaneously. When elongation of some DNA
molecules is stopped accidentally, it creates disturbance
of the cluster’s fluorescent signal, resulting sequencing
errors. Such errors accumulate during the process of sequencing,
and cause reads quality decreasing while the
length grows. Besides, deficiency on sequencing chips
and the existence of air bubbles on chip surface can
cause failure on reads from a whole tile. To get reliable
result in downstream analysis, it is necessary to remove
low quality reads avoiding mismatches in read mapping,
and false paths during genome assembly