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A feeding studywas conducted to evaluate the effects of an exogenous phytic acid diet on the dietary magnesium
(Mg) requirement for soft-shelled turtles. Juvenile soft-shelled turtles, Pelodiscus sinensis, were fed diets that
contained 36, 160, 357, 630, 947, 1249, or 1560 mg/kg Mg and 11 g/kg of phytic acid for 8 weeks. Weight gain
of turtles generally increased as dietary Mg increased up to approximately 630 mg/kg followed by a plateau
stage till 1560 mg/kg. The plasma alkaline phosphatase (ALP) activity, carapace strength and concentration of
Mg in the tissue generally increased as dietary Mg concentrations increased from 36 to 947 mg/kg. However,
increases inMg beyond 947 mg/kg caused the Mg concentrations in the tissue and carapace strength to remain
constant. When 11 g/kg of phytic acid was added to the diet and broken-line analysis used weight gain as the
indicator, the dietary Mg requirements for soft-shelled turtleswas estimated at 715 mg/kg. This is a 10% increase
from the requirement reported for turtles fed a phytate-free diet. A dietary intake of 970 mg Mg/kg is recommended
to further enhance carapace strength and plasma ALP activity in soft-shelled turtles.
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