The compound was obtained as pale w

The compound was obtained as pale white amorphous powder (yield: 117 mg, 0.68%). The FTIR peaks determined the bonds relevant to alcoholic O–H stretching (3267 cm−1), =C–H stretching (3010 cm−1), –C–H stretching (2975 cm−1), –C=O stretching (1605 cm−1), –C=C stretching (1506 cm−1), –C–H bending (1154 cm−1), C–O stretching (1315 cm−1), =C–H bending (1030 cm−1), and O–H bending (993 cm−1). 1H NMR (CDCl3, 400 MHz): δ (ppm) 3.91 (3H, , 4′-OMe), 3.89 (3H, , 7-OMe), 3.87 (3H, , 3′-OMe), 6.49 (, 1H, OH), 6.64 (, = 6.63 Hz,  1H, H6), 6.898 (, 1H, H8), 6.99 (, = 6.3 Hz, 1H, H2′), 7.52 (, = 6.3 Hz,  1H, H5′), 8.178 (s, 1H, H6′); 13C NMR (CDCl3, 100 MHz): δ (ppm) 171.61 (C-4), 144.30 (C-4′), 125.69 (C-1′), 162.87 (C-2), 161.77 (C-3′) 156.82 (C-5), 130.87 (C-6′), 114.28 (C-5′), 137.29 (C-9), 103.92 (C-2′), 157.74 (C-7), 98.92 (C-6), 98.19 (C-8), 111.47 (C-3), 113.58 (C-10), 54.51 (7-OMe), 54.76 (3′-OMe), 54.88 (4′-OMe) (Figures S1 and S2 of the Supplementary Information available online at http://dx.doi.org/10.1155/2014/549836). The compound exhibits a molecular weight of 329 (ESI-MS; 351.084 [M + Na]+) with an elemental formula of C18H16O6 (Figure 3). From these spectral interpretations, the isolated compound has been characterized as 2-(3, 4-dimethoxyphenyl)-5-hydroxy-7-methoxy-4H-chromen-4-one (5-hydroxy-3′, 4′, 7-trimethoxyflavone) (Figure 4), which agreed with the data reported [34].

3.2. Antioxidant Activities of Methanol Extract and Isolated Compound from Ethyl Acetate Fraction (EAF)

Flavonoids, a major group of polyphenols, are considered to be the active principles in diverse medicinal plants and have been reported to possess numerous pharmacological properties. The most essential biological activity of flavonoids is mainly due to their antioxidant property by acting as radical scavengers, hydrogen donors, reducing agents, and peroxidation inhibitors [35]. Previous studies reported that the pharmacological effects of L. nodiflora, such as antioxidant, diuretic, anti-inflammatory, and antimicrobial activities, were recognized due to the presence of the phenol and flavonoid compounds [33, 36]. In the present study, the isolated compound was identified as flavone and its antioxidant activities were examined by using various in vitro antioxidant models.
3.2.1. DPPH Radical Scavenging Effect

The substances are considered to be antioxidants, when they are capable of reducing the stable DPPH radical (purple) to the nonradical form DPPH-H (yellow), and thus they act as radical scavengers due to their hydrogen donating abilities [37]. The results of DPPH scavenging activity of all test samples are presented in Figure 5(a). The scavenging activity of methanolic extract, compound, and BHT increased with an increase in sample concentration (10–50 μg/mL). The highest DPPH scavenging activity for extract, compound, and BHT was found to be 79.35%, 72.66%, and 86.09%, respectively, at 50 μg/mL. It should be noted that the scavenging activity of compoundwas found to be close to the extract. The IC50 values of scavenging activity on DPPH radical of extract and BHT are given in Table 1, whereas, for compound, it is found to be 27 μg/mL. From these obtained data, the extract and compound were considered as an effective free-radical inhibitor as well as the primary antioxidants, which may limit free-radical damage that takes place in the body.

Results (Indonesian) 1:

Senyawa ini diperoleh sebagai bubuk amorf putih pucat (menghasilkan: 117 mg, 0,68%). Puncak FTIR yang ditentukan obligasi yang relevan untuk O-H beralkohol peregangan (3267 cm−1), = C-H peregangan (3010 cm−1), peregangan-C-H (2975 cm−1), -C = O peregangan (1605 cm−1),-C = C peregangan (cm−1 1506), membungkuk-C-H (1154 cm−1), C-O peregangan (cm−1 1315), = C-H membungkuk (1030 cm−1), dan O-H membungkuk (993 cm−1). 1H NMR (CDCl3, 400 MHz): δ (ppm) 3.91 (3H, 4 '-OMe), 3.89 (3H, 7-OMe), 3.87 (3H, 3 '-OMe), 6,49 (, 1H, OH), 6.64 (, = 6.63 Hz, 1H, H6), 6.898 (, 1H, H8), 6.99 (, = 6.3 Hz, 1H, H2′), 7.52 (, = 6.3 Hz, 1H, H5′), 8.178 (s, 1H, H6′); 13C NMR (CDCl3, 100 MHz): δ (ppm) 171.61 (C-4), 144.30 (C-4 '), 125.69 (C-1 '), 162.87 (C-2), 161.77 (C-3 ') 156.82 (C-5), 130.87 (C-6 '), 114.28 (C-5 '), 137.29 (C-9), 103.92 (C-2 '), 157.74 (C-7), 98.92 (C-6), 98.19 (C-8), 111.47 (C-3), 113.58 (C-10), sejumlah 54,51 (7-OMe), 54,76 (3 '-OMe), 54.88 (4 '-OMe) (angka S1 dan S2 informasi tambahan yang tersedia online di http://dx.doi.org/10.1155/2014/549836). Senyawa pameran berat molekul 329 (ESI-MS; 351.084 [M + Na] +) dengan formula unsur dari C18H16O6 (gambar 3). Dari interpretasi spektral, senyawa terisolasi telah ditandai sebagai 2-(3, 4-dimethoxyphenyl)-5-hydroxy-7-methoxy-4H-chromen-4-one (5-hidroksi-3 ' 4 ', 7-trimethoxyflavone) (gambar 4), yang setuju dengan data yang dilaporkan [34].3.2. aktivitas antioksidan metanol ekstrak dan diisolasi senyawa dari fraksi etil asetat (EAF)Flavonoid, sekelompok besar polifenol, dianggap prinsip-prinsip aktif dalam beragam tanaman obat dan telah dilaporkan memiliki banyak sifat farmakologis. Paling penting kegiatan biologis flavonoid adalah terutama karena antioksidan properti mereka dengan bertindak sebagai radikal pemulung, hidrogen donor, agen mengurangi dan peroxidation inhibitor [35]. Studi sebelumnya dilaporkan bahwa efek farmakologis dari L. nodiflora, seperti aktivitas antioksidan, diuretik, anti-inflamasi dan antimikroba, yang diakui karena adanya fenol dan flavonoid senyawa [33, 36]. Dalam penelitian ini, senyawa terisolasi diidentifikasi sebagai Flavon dan aktivitas antioksidan yang diperiksa dengan menggunakan berbagai model secara in vitro antioksidan.3.2.1. DPPH radikal pemulungan efekZat yang dianggap sebagai antioksidan, ketika mereka mampu mengurangi DPPH stabil yang radikal (ungu) untuk membentuk nonradical DPPH-H (kuning), dan dengan demikian mereka bertindak sebagai karena mereka hidrogen yang menyumbangkan kemampuan [37]. Hasil DPPH pemulungan aktivitas sampel uji semua disajikan dalam gambar 5. Aktivitas scavenging methanolic ekstrak, senyawa dan BHT meningkat dengan peningkatan konsentrasi sampel (10-50 μg/mL). DPPH tertinggi yang mengais-ngais aktivitas ekstrak, senyawa, dan BHT ditemukan untuk menjadi 79.35%, 72.66% dan 86.09%, masing-masing, di 50 μg/mL. Perlu dicatat bahwa aktivitas scavenging compoundwas ditemukan untuk menjadi dekat dengan ekstrak. Nilai-nilai IC50 pemulungan aktivitas di DPPH radikal ekstrak dan BHT diberikan dalam tabel 1, sedangkan untuk senyawa, hal ini ditemukan untuk menjadi 27 μg/mL. Dari ini diperoleh data, ekstrak dan senyawa dianggap sebagai inhibitor radikal bebas yang efektif serta antioksidan utama, yang dapat membatasi kerusakan radikal bebas yang terjadi dalam tubuh.