Shanghai Precision Instruments, Shanghai, China) which located in a balance room. Before filtration, the washing was hand-shaken for several seconds to resuspend all washed particles.The filtrationwas carried using a 47mm glass filter funnel with stopper support assembly (Millipore Corp., Bedford, MA, USA) connected to a vacuum pump
(SHB-III; Greatwall Scientific Industrial and Trade, Co., Ltd., Zhengzhou, China).Water-soluble particles and the particles with a diameter of