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The most distal 15 mm of the tail tip of each lizard was excised using a sterilized scalpel. Individual lizards were released at their site of capture after tissue sampling. Tissue samples were pre- served in 95% ethanol before they were deposited at Hangzhou Normal University and assigned voucher numbers identified by locality-haplotype numbers. Total DNA was extracted using stan- dard phenol–chloroform methods (Sambrook et al., 1989), and stored at
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