Analytical ProceduresBoth UV-VIS sp

Analytical Procedures
Both UV-VIS spectrophotometric method and RP-HPLC
methods were used in the present investigation. UV-VIS
spectrophotometric method (UV-Double Beam Spectrophotometer,
UV-1800, Shimadzu, Japan) based on the measurement of
absorbance at 256nm in a methanol stock solution was used. A
reverse phase HPLC system was used for the analysis of OXC in
different samples. Chromatographic separation was performed on a
Shimadzu Prominence HPLC system equipped with LC 10 AVP
binary pumps, DGU 20A Degasser, M20 A PDA detector and LC
10ATVP auto sampler with 200μL loop volume.

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Analytical ProceduresBoth UV-VIS spectrophotometric method and RP-HPLCmethods were used in the present investigation. UV-VISspectrophotometric method (UV-Double Beam Spectrophotometer,UV-1800, Shimadzu, Japan) based on the measurement ofabsorbance at 256nm in a methanol stock solution was used. Areverse phase HPLC system was used for the analysis of OXC indifferent samples. Chromatographic separation was performed on aShimadzu Prominence HPLC system equipped with LC 10 AVPbinary pumps, DGU 20A Degasser, M20 A PDA detector and LC10ATVP auto sampler with 200μL loop volume.

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Prosedur analitis
Kedua UV-VIS metode dan RP-HPLC spektrofotometri
metode yang digunakan dalam penyelidikan ini. UV-VIS
metode spektrofotometri (UV-Double Beam Spectrophotometer,
UV-1800, Shimadzu, Jepang) berdasarkan pengukuran
absorbansi pada 256nm dalam larutan stok metanol digunakan. Sebuah
sistem HPLC fase terbalik digunakan untuk analisis OXC di
sampel yang berbeda. Pemisahan kromatografi dilakukan pada
sistem Shimadzu Prominence HPLC yang dilengkapi dengan LC 10 AVP
pompa biner, DGU 20A Degasser, detektor M20 A PDA dan LC
10ATVP auto sampler dengan 200μL Volume lingkaran.

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