Pineapple shell contained high amount of cellulose (37.68±6.97%) and sugar production (36.25±2.87
g/L) after hot water pretreatment and enzymatic hydrolysis, respectively. The enzyme hydrolysate was
utilized as fermentation medium, with no nutritional addition to produce ethanol and hydrogen by S.
cerevisiae and E. aerogenes. The maximum yield of ethanol (9.69 g/L) was achieved after 72 h of S.
cerevisiae cultivation with no hydrogen production. Lower production of ethanol (1.38 g/L) was observed
after 72 h by E. aerogenes. However, E. aerogenes yielded high hydrogen production (1,416 mL/L) after
12 h of cultivation. To improve the concentration of ethanol and hydrogen, the immobilized cell
technique was applied. The result indicated that biofuel production was increased significantly by
immobilized cell technique. In this study, pineapple peel was proved as one of the novel and potential raw
material for biofuel production. However, optimization of substrate concentration and other
environmental conditions will require for an industrial application