DPPH Radical Scavenging Assay. The

DPPH Radical Scavenging Assay. The DPPH radical
scavenging activities of methanol extract, EAF, BF, and WF
were tested according to Yamaguchi et al. [27]. Briefly, 0.2mL
of the sample solutions of different concentrations was added
to 1mL of 0.1mM of freshly prepared DPPH solution. The
reaction mixtures were shaken vigorously and absorbance at
517 nm was determined after 20min at room temperature.
Control sample was prepared containing the same volume
without test compounds or reference antioxidants, while
DMSO was used as blank. The reference antioxidant BHT
was used as the positive control in all the assays. The
radical scavenging activity was measured as a decrease in the
absorbance of DPPH∙ and calculated as follows:

Results (Indonesian) 2:

DPPH Radical Scavenging Assay. DPPH radikal
aktivitas pemulung ekstrak metanol, EAF, BF, dan WF
diuji sesuai dengan Yamaguchi et al. [27]. Secara singkat, 0.2mL
dari solusi sampel konsentrasi yang berbeda ditambahkan
ke 1mL 0.1mm baru disiapkan solusi DPPH. The
campuran reaksi terguncang keras dan absorbansi pada
517 nm ditentukan setelah 20 menit pada suhu kamar.
Kontrol sampel disusun mengandung volume yang sama
tanpa senyawa uji atau antioksidan referensi, sementara
DMSO digunakan sebagai kosong. Antioksidan referensi BHT
digunakan sebagai kontrol positif di semua tes. The
aktivitas antioksidan diukur sebagai penurunan
absorbansi DPPH ∙ dan dihitung sebagai berikut: