Improvements to thin layer chromato

Improvements to thin layer chromatography (TLC) analysis can be made easily and cheaply by the application of digital colour
photography and image analysis. The combined technique, digitally enhanced TLC (DE-TLC), is applicable to the accurate
quantification of analytes in mixtures, to reaction monitoring and to other typical uses of TLC. Examples are given of the
application of digitally enhanced TLC to: the deuteromethylations of theophylline to [methyl2H3]caffeine and ofumbelliferone
to [2H3]7-methoxycoumarin; the selection of tertiary amine bases in deuterodechlorination reactions; stoichiometry
optimisation in the borodeuteride reduction of quinizarin (1,4-dihydroxyanthraquinone) and to the assessment of xanthophyll
yields in Lepidium sativum seedlings grown in deuterated media.

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Improvements to thin layer chromatography (TLC) analysis can be made easily and cheaply by the application of digital colourphotography and image analysis. The combined technique, digitally enhanced TLC (DE-TLC), is applicable to the accuratequantification of analytes in mixtures, to reaction monitoring and to other typical uses of TLC. Examples are given of theapplication of digitally enhanced TLC to: the deuteromethylations of theophylline to [methyl2H3]caffeine and ofumbelliferoneto [2H3]7-methoxycoumarin; the selection of tertiary amine bases in deuterodechlorination reactions; stoichiometryoptimisation in the borodeuteride reduction of quinizarin (1,4-dihydroxyanthraquinone) and to the assessment of xanthophyllyields in Lepidium sativum seedlings grown in deuterated media.

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Perbaikan analisis kromatografi lapis tipis (TLC) dapat dibuat dengan mudah dan murah dengan aplikasi warna digital
fotografi dan analisis citra. Teknik gabungan, ditingkatkan secara digital TLC (DE-TLC), berlaku untuk akurat
kuantifikasi analit dalam campuran, untuk pemantauan reaksi dan kegunaan khas lain dari TLC. Contoh diberikan dari
penerapan digital yang disempurnakan TLC kepada: deuteromethylations teofilin untuk [? Metil 2H3] kafein dan ofumbelliferone
untuk [2H3] 7-methoxycoumarin; pemilihan basis amina tersier dalam reaksi deuterodechlorination; stoikiometri
optimasi dalam pengurangan borodeuteride dari quinizarin (1,4-dihydroxyanthraquinone) dan penilaian xantofil
hasil di bibit Lepidium sativum tumbuh di media yang deuterated.

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