The contents of total ASA and total

The contents of total ASA and total glutathione were determined
by dissolving 0.1 g homogenates in 1ml of 5% mphosphporic
acid solution. The extract was then centrifuged
at 13,000 × g for 10 min at 4 ◦C. The supernatant was used
for total ASA and total glutathione assay. Both total ASA
and the reduced ASA contents were determined spectrophotometrically
at 525 nm using different reacting solutions; the
former was a potassium phosphate buffer (pH 7.4), dithiothreitol
and N-ethlmaleimide, the latter was trichloroacetic
acid, o-phosphoric acid,
-dipyridyl in 70% alcohol and
FeCl3 [38]. Total ASA and the reduced ASA contents were
calculated from a standard curve plotted from a known concentration
of ASA. In addition to total ASA and reduced
ASA contents, we also calculated the ratio of oxidized ASA
to reduced ASA as an indication of the redox state of ascorbate
in the roots. The content of oxidized ASA (Vitamin
C) was calculated by subtracting the reduced ASA content
from the total ASA content.

Results (English) 3:

内容总抗坏血酸和总谷胱甘肽的测定
溶解0.1克的组织匀浆中的5% mphosphporic
酸溶液1ml。提取后离心
在13000×G在4◦C.上清液用
总抗坏血酸和总谷胱甘肽含量为10分钟。分光光度法测定
都总抗坏血酸和抗坏血酸的含量，降低
在525 nm处使用不同的反应溶液的
；前一个磷酸钾缓冲液（pH 7.4），二硫苏糖醇
和n-ethlmaleimide，后者是三氯
酸，邻-磷酸，
- 70%酒精和
FeCl3 [ 38联吡啶]。总抗坏血酸和还原AsA含量
从标准曲线绘制从一个已知浓度的
ASA计算。除了总抗坏血酸和还原
ASA的含量，还计算了AsA氧化
减少ASA抗坏血酸的
根中的氧化还原状态的显示比例。氧化抗坏血酸（维生素C含量
）是通过从总抗坏血酸含量降低，抗坏血酸含量减去
计算。