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in the absence of fermentable glucosa or lactose ,some microorganisms are capable of using citrate as a carbon for their energy . this ability depends of the presence of citrate permease that facilitates the transport of cirate in the cell. citrate is the first major intermediate in the crebs cycle and is produced by condensation of active acetyl with oxaloacetic acid. citrate is acted on by the enzym citrate ,which produced oxaloacetid acid and acetate.following incubation
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