Results (
Thai) 1:
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The collected eggs were immediately used for artificial fertilization.
Two grams of eggs were inseminated with 1 mL of the pre-diluted milt
(sperm motility>50%) and then dispersed in 100 mL of seawater. Three
milliliters of this seawater containing about 120 eggs was collected with
a pipet and used for assessing the hatchability and the production of
first-feeding larvae by an individual rearing method using 48-well tissue
culturemicroplates (Unuma et al., 2004). Rearingwater, salinity ofwhich
was adjusted to 34‰ with NaCl (Okamoto et al., 2009), was added
with bovine serumalbumin (1 μg/mL) to prevent surface tension-related
death and penicillin G potassium (50 IU/mL) and streptomycin sulfate
(0.05 mg/mL) to prevent bacterial proliferation (Unuma et al., 2011b).
Microplates stocked with the inseminated eggs were maintained at
25 °C (Kurokawa et al., 2008) in an incubator with wet chambers.
Hatched larvae were counted 2 days post‐hatch (DPH)
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