MATERIALS AND METHODSShimadzu UV sp

MATERIALS AND METHODS
Shimadzu UV spectrophotometer 1700 and JascoV-630
spectrophotometer with 1 cm matched quartz cells was used for all
spectral and absorbance measurements. Pure drug was procured
from Yarrow Chem Products Ltd, Mumbai as a gift sample. Basic
apparatus like calibrated volumetric flasks, pipette, beakers and
graduated pipettes were used.
Experimental
Preparation of stock solutions
100 mg standard Salbutamol Sulphate was weighed and transferred
to a 100 ml volumetric flask and dissolved in distilled water. The
flask was shaken and volume was made up to the mark with distilled
water to get a concentration of 1000 μg/ml (Stock solution A). From
this stock solution A, 10 ml solution was pipetted out and placed
into 100 ml volumetric flask. The volume was made up to mark with
distilled water to get a concentration of 100μg/ml (Stock solution B).
Preparation of reagents and chemicals
a) Preparation of 1N NaOH
4 gm of NaOH was dissolved in 100 ml of distilled water.
b) Preparation of 2N FCR
50 ml of standard phenol reagent was taken and diluted to 100 ml
with distilled water.
Determination of [λmax Selection of analytical wavelength]
To a 10 ml volumetric flask, 1 ml of drug solution (100μg/ml) from
Stock solution B was added. To the flask 0.8 ml of 2N FCR solution and 6 ml of distilled water was added. The volumetric flask was shaken well for at least 5 min followed by addition of 1.5 ml of 1N NaOH solution. The volume was made up to the mark and stored for 15 min in the dark. The absorbance reading was taken against the blank. The solution was scanned in UV spectrophotometer between 800-400 nm. Absorption spectra obtained showed the absorption maxima [λmax
Preparation of Standard curve ] 723 nm, which was selected as wavelength for analytical measurements. Absorption maxima obtained is given in fig 2

Results (Indonesian) 2:

BAHAN DAN METODE
Shimadzu UV spektrofotometer 1700 dan JascoV-630
spektrofotometer dengan 1 cm dicocokkan sel quartz digunakan untuk semua
pengukuran spektral dan absorbansi. Obat murni diperoleh
dari Yarrow Chem Produk Ltd, Mumbai sebagai sampel hadiah. Dasar
aparat seperti termos dikalibrasi volumetrik, pipet, gelas dan
pipet lulus digunakan.
Eksperimental
Pembuatan larutan stok
100 mg standar Salbutamol sulfat ditimbang dan dipindahkan
ke 100 ml labu ukur dan dilarutkan dalam air suling. The
termos terguncang dan volume dibuat sampai tanda dengan suling
air untuk mendapatkan konsentrasi 1000 mg / ml (larutan Stock A). Dari
larutan stok ini A, 10 ml larutan dipipet keluar dan ditempatkan
menjadi 100 labu volumetrik ml. Volume dibuat untuk menandai dengan
air suling untuk mendapatkan konsentrasi 100μg / ml (Stock solusi B).
Persiapan reagen dan bahan kimia
a) Penyusunan 1N NaOH
4 gram NaOH dilarutkan dalam 100 ml air suling.
B) persiapan 2N FCR
50 ml standar pereaksi fenol diambil dan diencerkan sampai 100 ml
dengan air suling.
Penentuan [λmax Pemilihan panjang gelombang analitis]
untuk 10 ml labu ukur, 1 ml larutan obat (100μg / ml) dari
larutan Stock B ditambahkan. Untuk termos 0,8 ml larutan 2N FCR dan 6 ml air suling ditambahkan. Labu volumetrik dikocok dengan baik selama sedikitnya 5 menit diikuti dengan penambahan 1,5 ml larutan 1N NaOH. Volume dibuat sampai tanda dan disimpan selama 15 menit dalam gelap. Absorbansi membaca yang diambil terhadap kosong. Larutan tersebut dipindai di spektrofotometer UV antara 800-400 nm. Spektrum penyerapan diperoleh menunjukkan maxima penyerapan [λmax
Pembuatan kurva Standard] 723 nm, yang terpilih sebagai panjang gelombang untuk pengukuran analitis. Penyerapan maxima diperoleh diberikan dalam ara 2