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Because of the structure diversity of alkaloids, there is no single method of their extraction from nature raw materials. Most methods exploit the property of most alkaloids to be soluble in organic solvents but not water. Most alkaloids are present in the raw plants in the form of salt of organic acids. Usually inorganic acidic extraction is used, so that the organic acid of alkaloids salt is replaced inorganic acid salt, and increasing its solubility. The advantage of acidic extraction is changing alkaloids molecules into small molecular organic acid salts, increasing the solubility in water, Each monomer of alkaloids often has different alkaline, we can use the pH gradient extraction to separate. For example, separating A and D agent from the bark that is using their different alkaline, have the concentrated solution basified to leach in pH9-10. A agent was extracted of its low alkaline but B agent whose pH is higher than A agent. Precipitation method separate water-soluble impurities which remaining in the filtrate, in order to obtain higher purity water-soluble alkaloids or salts.
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