Well isolated colonies were transferred to BHI agar supplemented with 0.5% yeast extract and 1% glucose (supplemented BHI) and incubated at 37 C for 3e5 days.
Well isolated colonies were transferred to BHI agar supplemented with 0.5%yeast extract and 1% glucose (supplemented BHI) and incubated at37 C for 3e5 days.