In this study, the crude polysaccharides from soybean sprouts grown in the presence (PL) or absence (PD) of light were extracted using hot water method and further purified by DEAE-52 chromatography. Two fractions (PL3 and PD3) were obtained and SEM analysis indicated that they had distinct surface properties. PL3 and PD3, as suggested by FTIR analysis, showed similar functional group and monosaccharide composition while with slightly different molecular weight (126.61 and 111.94 kDa, respectively). In addition, when antioxidant abilities were examined by measuring DPPH radical scavenging activity and FRAP, PL3 exhibited stronger biological activity in vitro than PD3. Therefore, light illumination during germination is likely to have a significant effect on structural and functional properties of polysaccharides derived from soybean sprouts. These results supported that the green soybean sprout may be of potential health benefits due to its more active polysaccharides.