After 24 h, absorbance of aliquot of bathing solution was measured spectrophotometrically (UV-1700 PharmaSpec, Shimadzu, Japan) at 280 nm (A 280 initial).
After 24 h, absorbance of aliquot of bathingsolution was measured spectrophotometrically (UV-1700PharmaSpec, Shimadzu, Japan) at 280 nm (A 280 initial).